منابع مشابه
Squalene Epoxidase of Rat Liver*
The microsomal enzyme system from rat liver which catalyzes the epoxidation of squalene to 2,3-oxidosqualene (squalene epoxidase) has been investigated further with 10 ,ll-dihydrosqualene as the artacial substrate. Liver supernatant contributes two essential components to the epoxidase system, one heat stable and the other heat labile (YAMAMOTO, S., AND BLOCH, K. (1970) J. Biol. Chem. 245, 1670...
متن کاملMeasurement of squalene in human tissues and plasma: validation and application.
A method is described for accurate and reproducible measurement of squalene in plasma, feces, urine, bile, and tissue that depends on isolation by alumina column chromatography after mild saponification and on measurement by gas-liquid chromatography. Recoveries from all tissues exceeded 80% and from plasma 96%; losses were accurately corrected by appropriate additions of squalene as an overall...
متن کاملDiurnal variation of cholesterol precursors squalene and methyl sterols in human plasma lipoproteins.
Animal cholesterol synthesis shows a marked diurnal variation, a phenomenon, at the moment, not known to occur in man. Since cholesterol precursors in serum reflect overall cholesterol synthesis in many conditions, a 24-hr profile of squalene and methyl sterols was studied in plasma lipoproteins in order to demonstrate whether these cholesterol precursors could exhibit a diurnal cycling in heal...
متن کاملInfluence of squalene feeding on plasma leptin, testosterone & blood pressure in rats.
BACKGROUND & OBJECTIVES Obesity, hyperlipidaemia and hypercholesterolaemia are known risk factors in the pathogenesis of hypertension. Squalene has been shown to reduce serum cholesterol and triglycerides in dogs although its therapeutic use in high BP and obese patients has not been established. This study evaluates the effect of feeding high doses of squalene on plasma leptin, glucose, testos...
متن کاملStudies on Squalene Epoxidase of Rat Liver*
Rat liver microsomes previously heated to 50” for 5 mm accumulate 2,3-oxidosqualene on incubation with squalene. Squalene epoxidase activity can be assayed either with squalene and heated microsomes or with lO,ll-dihydrosqualene and intact microsomes. In common with other monooxygenases, the epoxidase requires TPNH and molecular oxygen. Both a soluble fraction of rat liver and microsomes are ne...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1964
ISSN: 0021-9738
DOI: 10.1172/jci105024